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ha epa1  (TargetMol)


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    TargetMol ha epa1
    The reduced cell surface exposure of Epa1 in the tir2-5 awp6-7 sextuple mutant. ( A ) Fluorescence-activated cell sorting (FACS) analysis <t>of</t> <t>HA-Epa1</t> fusion proteins on the cell surface in WT strain (black) and tir2-5 awp6-7 sextuple mutant (red). C. glabrata cells carrying HA-Epa1 from the TEF1 promoter were incubated in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% serum at 37°C in 5% CO 2 . Surface exposure was monitored by immunofluorescence after labeling with anti-HA antibody and fluoresceine isothiocyanate (FITC)-conjugated secondary goat anti-mouse antibody. No primary antibody control was included. Average fluorescence of the population was indicated. Plots are representative of data collected in two independent replicate experiments. ( B ) Western analysis of HA-Epa1 fusion protein in the cell wall fraction of WT and tir2-5 awp6-7 sextuple mutant cells incubated in DMEM medium with 10% serum at 37°C in 5% CO 2 . Band intensities were quantified and shown as the ratio of indicated protein vs tubulin. Representative plots of three independent experiments are shown. ( C ) Real-time quantitative reverse transcription PCR (qRT-PCR) analysis for EPA1 expression in C. glabrata cultured as described in ( B ). The expression levels of EPA1 were showed as means ± SD. n = 3 biologically independent experiments. ( D ) Adhesion assay on epithelial cell monolayers. C. glabrata cells of wild type and indicated mutant strains were inoculated with A549 cells in DMEM medium supplemented with 10% serum. After incubation for 4 h, non-adherent Candida cells were removed by washing with phosphate-buffered saline (PBS). The numbers of adherent Candida cells are represented as means ± SD from three independent experiments. ( E ) Cytotoxicity assays. A549 cell damage following treatment with wild type, tir2-5 quadruple mutant, awp6-7 double mutant, or tir2-5 awp6-7 sextuple mutant was determined after co-incubation for 24 h. Relative LDH activity (%) against A549 cells without C. glabrata cells was calculated. Mean data ± SD from three independent experiments was plotted. ( C–E ) Significance was determined using the two-tailed unpaired Student’s t -test. ns, nonsignificant. ** P < 0.01, *** P < 0.001.
    Ha Epa1, supplied by TargetMol, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ha epa1/product/TargetMol
    Average 92 stars, based on 1 article reviews
    ha epa1 - by Bioz Stars, 2026-05
    92/100 stars

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    1) Product Images from "An alteration in the expression of cell wall structural proteins increases cell surface exposure of adhesins to promote virulence in Candida glabrata"

    Article Title: An alteration in the expression of cell wall structural proteins increases cell surface exposure of adhesins to promote virulence in Candida glabrata

    Journal: mSphere

    doi: 10.1128/msphere.00910-24

    The reduced cell surface exposure of Epa1 in the tir2-5 awp6-7 sextuple mutant. ( A ) Fluorescence-activated cell sorting (FACS) analysis of HA-Epa1 fusion proteins on the cell surface in WT strain (black) and tir2-5 awp6-7 sextuple mutant (red). C. glabrata cells carrying HA-Epa1 from the TEF1 promoter were incubated in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% serum at 37°C in 5% CO 2 . Surface exposure was monitored by immunofluorescence after labeling with anti-HA antibody and fluoresceine isothiocyanate (FITC)-conjugated secondary goat anti-mouse antibody. No primary antibody control was included. Average fluorescence of the population was indicated. Plots are representative of data collected in two independent replicate experiments. ( B ) Western analysis of HA-Epa1 fusion protein in the cell wall fraction of WT and tir2-5 awp6-7 sextuple mutant cells incubated in DMEM medium with 10% serum at 37°C in 5% CO 2 . Band intensities were quantified and shown as the ratio of indicated protein vs tubulin. Representative plots of three independent experiments are shown. ( C ) Real-time quantitative reverse transcription PCR (qRT-PCR) analysis for EPA1 expression in C. glabrata cultured as described in ( B ). The expression levels of EPA1 were showed as means ± SD. n = 3 biologically independent experiments. ( D ) Adhesion assay on epithelial cell monolayers. C. glabrata cells of wild type and indicated mutant strains were inoculated with A549 cells in DMEM medium supplemented with 10% serum. After incubation for 4 h, non-adherent Candida cells were removed by washing with phosphate-buffered saline (PBS). The numbers of adherent Candida cells are represented as means ± SD from three independent experiments. ( E ) Cytotoxicity assays. A549 cell damage following treatment with wild type, tir2-5 quadruple mutant, awp6-7 double mutant, or tir2-5 awp6-7 sextuple mutant was determined after co-incubation for 24 h. Relative LDH activity (%) against A549 cells without C. glabrata cells was calculated. Mean data ± SD from three independent experiments was plotted. ( C–E ) Significance was determined using the two-tailed unpaired Student’s t -test. ns, nonsignificant. ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: The reduced cell surface exposure of Epa1 in the tir2-5 awp6-7 sextuple mutant. ( A ) Fluorescence-activated cell sorting (FACS) analysis of HA-Epa1 fusion proteins on the cell surface in WT strain (black) and tir2-5 awp6-7 sextuple mutant (red). C. glabrata cells carrying HA-Epa1 from the TEF1 promoter were incubated in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% serum at 37°C in 5% CO 2 . Surface exposure was monitored by immunofluorescence after labeling with anti-HA antibody and fluoresceine isothiocyanate (FITC)-conjugated secondary goat anti-mouse antibody. No primary antibody control was included. Average fluorescence of the population was indicated. Plots are representative of data collected in two independent replicate experiments. ( B ) Western analysis of HA-Epa1 fusion protein in the cell wall fraction of WT and tir2-5 awp6-7 sextuple mutant cells incubated in DMEM medium with 10% serum at 37°C in 5% CO 2 . Band intensities were quantified and shown as the ratio of indicated protein vs tubulin. Representative plots of three independent experiments are shown. ( C ) Real-time quantitative reverse transcription PCR (qRT-PCR) analysis for EPA1 expression in C. glabrata cultured as described in ( B ). The expression levels of EPA1 were showed as means ± SD. n = 3 biologically independent experiments. ( D ) Adhesion assay on epithelial cell monolayers. C. glabrata cells of wild type and indicated mutant strains were inoculated with A549 cells in DMEM medium supplemented with 10% serum. After incubation for 4 h, non-adherent Candida cells were removed by washing with phosphate-buffered saline (PBS). The numbers of adherent Candida cells are represented as means ± SD from three independent experiments. ( E ) Cytotoxicity assays. A549 cell damage following treatment with wild type, tir2-5 quadruple mutant, awp6-7 double mutant, or tir2-5 awp6-7 sextuple mutant was determined after co-incubation for 24 h. Relative LDH activity (%) against A549 cells without C. glabrata cells was calculated. Mean data ± SD from three independent experiments was plotted. ( C–E ) Significance was determined using the two-tailed unpaired Student’s t -test. ns, nonsignificant. ** P < 0.01, *** P < 0.001.

    Techniques Used: Mutagenesis, Fluorescence, FACS, Incubation, Modification, Immunofluorescence, Labeling, Control, Western Blot, Reverse Transcription, Quantitative RT-PCR, Expressing, Cell Culture, Cell Adhesion Assay, Saline, Activity Assay, Two Tailed Test



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    ha epa1  (TargetMol)
    92
    TargetMol ha epa1
    The reduced cell surface exposure of Epa1 in the tir2-5 awp6-7 sextuple mutant. ( A ) Fluorescence-activated cell sorting (FACS) analysis <t>of</t> <t>HA-Epa1</t> fusion proteins on the cell surface in WT strain (black) and tir2-5 awp6-7 sextuple mutant (red). C. glabrata cells carrying HA-Epa1 from the TEF1 promoter were incubated in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% serum at 37°C in 5% CO 2 . Surface exposure was monitored by immunofluorescence after labeling with anti-HA antibody and fluoresceine isothiocyanate (FITC)-conjugated secondary goat anti-mouse antibody. No primary antibody control was included. Average fluorescence of the population was indicated. Plots are representative of data collected in two independent replicate experiments. ( B ) Western analysis of HA-Epa1 fusion protein in the cell wall fraction of WT and tir2-5 awp6-7 sextuple mutant cells incubated in DMEM medium with 10% serum at 37°C in 5% CO 2 . Band intensities were quantified and shown as the ratio of indicated protein vs tubulin. Representative plots of three independent experiments are shown. ( C ) Real-time quantitative reverse transcription PCR (qRT-PCR) analysis for EPA1 expression in C. glabrata cultured as described in ( B ). The expression levels of EPA1 were showed as means ± SD. n = 3 biologically independent experiments. ( D ) Adhesion assay on epithelial cell monolayers. C. glabrata cells of wild type and indicated mutant strains were inoculated with A549 cells in DMEM medium supplemented with 10% serum. After incubation for 4 h, non-adherent Candida cells were removed by washing with phosphate-buffered saline (PBS). The numbers of adherent Candida cells are represented as means ± SD from three independent experiments. ( E ) Cytotoxicity assays. A549 cell damage following treatment with wild type, tir2-5 quadruple mutant, awp6-7 double mutant, or tir2-5 awp6-7 sextuple mutant was determined after co-incubation for 24 h. Relative LDH activity (%) against A549 cells without C. glabrata cells was calculated. Mean data ± SD from three independent experiments was plotted. ( C–E ) Significance was determined using the two-tailed unpaired Student’s t -test. ns, nonsignificant. ** P < 0.01, *** P < 0.001.
    Ha Epa1, supplied by TargetMol, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ha epa1/product/TargetMol
    Average 92 stars, based on 1 article reviews
    ha epa1 - by Bioz Stars, 2026-05
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    The reduced cell surface exposure of Epa1 in the tir2-5 awp6-7 sextuple mutant. ( A ) Fluorescence-activated cell sorting (FACS) analysis of HA-Epa1 fusion proteins on the cell surface in WT strain (black) and tir2-5 awp6-7 sextuple mutant (red). C. glabrata cells carrying HA-Epa1 from the TEF1 promoter were incubated in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% serum at 37°C in 5% CO 2 . Surface exposure was monitored by immunofluorescence after labeling with anti-HA antibody and fluoresceine isothiocyanate (FITC)-conjugated secondary goat anti-mouse antibody. No primary antibody control was included. Average fluorescence of the population was indicated. Plots are representative of data collected in two independent replicate experiments. ( B ) Western analysis of HA-Epa1 fusion protein in the cell wall fraction of WT and tir2-5 awp6-7 sextuple mutant cells incubated in DMEM medium with 10% serum at 37°C in 5% CO 2 . Band intensities were quantified and shown as the ratio of indicated protein vs tubulin. Representative plots of three independent experiments are shown. ( C ) Real-time quantitative reverse transcription PCR (qRT-PCR) analysis for EPA1 expression in C. glabrata cultured as described in ( B ). The expression levels of EPA1 were showed as means ± SD. n = 3 biologically independent experiments. ( D ) Adhesion assay on epithelial cell monolayers. C. glabrata cells of wild type and indicated mutant strains were inoculated with A549 cells in DMEM medium supplemented with 10% serum. After incubation for 4 h, non-adherent Candida cells were removed by washing with phosphate-buffered saline (PBS). The numbers of adherent Candida cells are represented as means ± SD from three independent experiments. ( E ) Cytotoxicity assays. A549 cell damage following treatment with wild type, tir2-5 quadruple mutant, awp6-7 double mutant, or tir2-5 awp6-7 sextuple mutant was determined after co-incubation for 24 h. Relative LDH activity (%) against A549 cells without C. glabrata cells was calculated. Mean data ± SD from three independent experiments was plotted. ( C–E ) Significance was determined using the two-tailed unpaired Student’s t -test. ns, nonsignificant. ** P < 0.01, *** P < 0.001.

    Journal: mSphere

    Article Title: An alteration in the expression of cell wall structural proteins increases cell surface exposure of adhesins to promote virulence in Candida glabrata

    doi: 10.1128/msphere.00910-24

    Figure Lengend Snippet: The reduced cell surface exposure of Epa1 in the tir2-5 awp6-7 sextuple mutant. ( A ) Fluorescence-activated cell sorting (FACS) analysis of HA-Epa1 fusion proteins on the cell surface in WT strain (black) and tir2-5 awp6-7 sextuple mutant (red). C. glabrata cells carrying HA-Epa1 from the TEF1 promoter were incubated in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% serum at 37°C in 5% CO 2 . Surface exposure was monitored by immunofluorescence after labeling with anti-HA antibody and fluoresceine isothiocyanate (FITC)-conjugated secondary goat anti-mouse antibody. No primary antibody control was included. Average fluorescence of the population was indicated. Plots are representative of data collected in two independent replicate experiments. ( B ) Western analysis of HA-Epa1 fusion protein in the cell wall fraction of WT and tir2-5 awp6-7 sextuple mutant cells incubated in DMEM medium with 10% serum at 37°C in 5% CO 2 . Band intensities were quantified and shown as the ratio of indicated protein vs tubulin. Representative plots of three independent experiments are shown. ( C ) Real-time quantitative reverse transcription PCR (qRT-PCR) analysis for EPA1 expression in C. glabrata cultured as described in ( B ). The expression levels of EPA1 were showed as means ± SD. n = 3 biologically independent experiments. ( D ) Adhesion assay on epithelial cell monolayers. C. glabrata cells of wild type and indicated mutant strains were inoculated with A549 cells in DMEM medium supplemented with 10% serum. After incubation for 4 h, non-adherent Candida cells were removed by washing with phosphate-buffered saline (PBS). The numbers of adherent Candida cells are represented as means ± SD from three independent experiments. ( E ) Cytotoxicity assays. A549 cell damage following treatment with wild type, tir2-5 quadruple mutant, awp6-7 double mutant, or tir2-5 awp6-7 sextuple mutant was determined after co-incubation for 24 h. Relative LDH activity (%) against A549 cells without C. glabrata cells was calculated. Mean data ± SD from three independent experiments was plotted. ( C–E ) Significance was determined using the two-tailed unpaired Student’s t -test. ns, nonsignificant. ** P < 0.01, *** P < 0.001.

    Article Snippet: Cell wall proteins were then released from the washed cell wall material by treatment with Endo-1,3-β-glucanase (TargetMoI) and probed for HA-Epa1 using anti-HA antibody (MBL).

    Techniques: Mutagenesis, Fluorescence, FACS, Incubation, Modification, Immunofluorescence, Labeling, Control, Western Blot, Reverse Transcription, Quantitative RT-PCR, Expressing, Cell Culture, Cell Adhesion Assay, Saline, Activity Assay, Two Tailed Test